ELK Biotechnology

Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit

Product Code:
 
ELK0755
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK0755-48T48T£298.00
Quantity:
ELK0755-96T96T£390.00
Quantity:
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Further Information

Alternative Names:
15-Deoxy-delta 12,14-prostaglandin J2
Assay Length:
2.5h
Assay Type:
Competitive Inhibition
Detection Range:
3.13-200 ng/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
1.21 ng/mL
Standard:
200 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 15d-PGJ2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 15d-PGJ2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.