ELK Biotechnology

Human ADH4 (Alcohol Dehydrogenase 4) ELISA Kit

Product Code:
 
ELK9860
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Human
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9860-48T48T£298.00
Quantity:
ELK9860-96T96T£390.00
Quantity:
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Further Information

Alternative Names:
Ac1002; ADH 4; ADH-1; ADH-2; ADH2; Adh4; ADH4_HUMAN; Alcohol dehydrogenase 4 (class II) pi polypeptide; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain; Alcohol dehydrogenase pi isozyme ADH class II; Aldehyde reductase; Class II alcohol dehydrogenase 4 pi subunit;
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.79-50 ng/mL
Sample Type:
Serum, plasma and other biological fluids.
Sensitivity:
0.28 ng/mL
Standard:
50 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADH4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADH4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADH4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human ADH4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P08319