NSJ Bioreagents

NSE Antibody / Neuron Specific Enolase

Product Code:
 
NSJ-V4230
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ENO/8253R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the NSE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human pancreas tissue with NSE antibody (clone ENO/8253R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human pancreas tissue with NSE antibody (clone ENO/8253R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

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CodeSizePrice
NSJ-V4230-20UG20 ug£264.00
Quantity:
NSJ-V4230-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Description:
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant fragment of human NSE gamma (within amino acids 234-434) was used as the immunogen for the NSE antibody.
Limitation:
This NSE antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P09104

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