NSJ Bioreagents

Phosphorylated JNK Antibody / pThr183/pTyr185

Product Code:
 
NSJ-R20476
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM464
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant phosphorylated JNK antibody at -20°C.
1 / 3
IHC staining of FFPE human colon tissue with recombinant phosphorylated JNK antibody at 1:100.
2 / 3
IHC staining of FFPE human colon cancer tissue with recombinant phosphorylated JNK antibody at 1:100.
3 / 3
Western blot testing of the lysate of human HeLa cells treated (+) or nontreated (-) with UV, using recombinant phosphorylated JNK antibody at a 1:200 dilution.
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IHC staining of FFPE human colon tissue with recombinant phosphorylated JNK antibody at 1:100.
IHC staining of FFPE human colon cancer tissue with recombinant phosphorylated JNK antibody at 1:100.
Western blot testing of the lysate of human HeLa cells treated (+) or nontreated (-) with UV, using recombinant phosphorylated JNK antibody at a 1:200 dilution.

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NSJ-R20476-0.1ML100 ul£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Western blot: 1:200-1:400
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phosphorylated JNK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human JNK1, JNK2, and JNK3 only when phosphorylated at Thr183 and Tyr185.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human Phospho-JNK1/2/3 (Thr183/Tyr185) was used as the immunogen for the recombinant phosphorylated JNK antibody.
Limitation:
This recombinant phosphorylated JNK antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P45983, P45984, P53779

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