anti-NS5B (HCV) mAb (5B-3B1)
| Code | Size | Price |
|---|
| AG-20B-0002-C100 | 100 ug | £300.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5B-3B1
Regulatory Status: RUO
Target Species: Hepatitis C virus (HCV)
Application: Western Blot (WB)
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Nonstructural Protein 5B
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).
Long Description:
Monoclonal Antibody. Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a. Isotype: Mouse IgG2b. Clone: 5B-3B1. Applications: WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
NCBI, Uniprot Number:
N/A
Package Type:
Plastic Vial
Product Description:
The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002) | Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells: R.W. Hardy, et al.; J. Virol. 77, 2029 (2003) | Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs: G. Randall, et al.; PNAS 100, 235 (2003) | Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005) | Quantitative analysis of the hepatitis C virus replication complex: D. Quinkert, et al.; J. Virol. 79, 13594 (2005) | Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3: Y.V. Svitkin, et al.; J. Virol. 79, 6868 (2005)