anti-Tubulin-GTP mAb (rec.) (MB11)
| Code | Size | Price |
|---|
| AG-27B-0009-C100 | 100 ug | £360.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Antibody Isotype: IgG2λ
Antibody Clonality: Recombinant Antibody
Antibody Clone: MB11
Regulatory Status: RUO
Target Species:
- Drosophila (fruit fly)
- Human
- Mouse
- Rat
Application: Immunocytochemistry (ICC)
Shipping:
-20°C
Storage:
-20°C
Documents
Further Information
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Full length GTP-lambda-S loaded tubulin from pig brain.
Long Description:
Recombinant Antibody. Recognizes human, mouse, rat and drosophila tubulin-GTP. Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: MB11. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
NCBI, Uniprot Number:
N/A
Other data:
anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Package Type:
Plastic Vial
Product Description:
anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human, mouse, rat and drosophila tubulin-GTP.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
References
Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues: A. Dimitrov, et al.; Science 322, 1353 (2008) | Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport: T. Nakata, et al.; J. Cell Biol. 194, 245 (2011) | Kinesin-3 Responds to Local Microtubule Dynamics to Target Synaptic Cargo Delivery to the Presynapse: P. Guedes-Dias, et al.; Curr. Biol. 29, 268 (2019) | Molecular understanding of label-free second harmonic imaging of microtubules: V. Van Steenbergen, et al.; Nat. Commun. 10, 3530 (2019) | Golgi?associated microtubules are fast cargo tracks and required for persistent cell migration: H. Hao, et al.; EMBO Rep. 21, e48385 (2020) | Tubulin binding potentially clears up Bortezomib and Carfilzomib differential neurotoxic effect: A. Malacrida, et al.; Nature Sci. Rep. 11, 10523 (2021)