JaICA

Nuclease P1 (from Penicillium citrinum)

Product Code:
 
JAI-OPNUCP1
Product Group:
 
Recombinant Proteins
Supplier:
 
JaICA
Regulatory Status:
 
RUO
Shipping:
 
BLUE ICE
Storage:
 
+4°C
 

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CodeSizePrice
JAI-OPNUCP1-500Units500 Units£1,748.00
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This product comes from: Switzerland.
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Further Information

Biological Activity:
3?-5?-Phosphodiesterase: One unit will liberate ~1.0µmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3?-Nucleotidase: One unit will hydrolyze ~4.0µmol of orthophosphate from 3?-AMP per min at pH 7.2 at 37°C.
EClass:
32160000
Form (Short):
liquid
Formulation:
Lyophilized.
Long Description:
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5'-phosphates and 5'-phospho-oligonucleotides. The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.
Molecular Weight:
42-50 kDa
Other data:
The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8. Optimal pH: 5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP) Heat Stability: Stable below 60°C at pH 5.3 for 30 minutes.
Package Type:
Plastic Vial
Product Description:
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5?-phosphates and 5?-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0.
Sequence:
A zinc dependent glycoprotein consisting of 270 amino acid residues.
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 1 year after receipt when stored at +4°C.

References

The interactions of dopamine and oxidative damage in the striatum of patients with neurodegenerative diseases: H. Li, et al.; J. Neurochem. 152, 235 (2020)