AdipoGen Life Sciences

NAPRTase (human) (rec.) (His)

Product Code:
 
AG-40T-0105
Product Group:
 
Recombinant Proteins
Antibody Clonality:
 
Enzyme
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Dry Ice
Storage:
 
-80°C
 

No additional charges, what you see is what you pay! *

CodeSizePrice
AG-40T-0105-C05050 ug£357.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Show All

Further Information

Alternate Names/Synonyms:
Nicotinate Phosphoribosyltransferase; FHA-HIT-interacting Protein; FHIP; EC 2.4.2.11
Biological Activity:
0.043µmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 µM PRPP, 100 µM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 µM, 25°C, spectrophotometrical monitoring.
Concentration:
0.5mg/ml (Lot specific)
EClass:
32160000
Endotoxin:
N/A
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Human NAPRTase is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT. Purity: >98% (SDS-PAGE). Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
Molecular Weight:
~58.0kDa
NCBI, Uniprot Number:
Q6XQN6
Package Type:
Plastic Vial
Product Description:
Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
Purity:
>98% (SDS-PAGE)
Sequence:
Human NAPRTase is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.

References

Characterization of human nicotinate phosphoribosyltransferase: Kinetic studies, structure prediction and functional analysis by site-directed mutagenesis: L. Galassi, et al.; Biochimie 94, 300 (2012)