ASC Antibody
Product Code:
PSI-2287
PSI-2287
Host Type:
Rabbit
Rabbit
Antibody Isotype:
IgG
IgG
Antibody Clonality:
Polyclonal
Polyclonal
Regulatory Status:
RUO
RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-2287-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-2287-0.1mg | 0.1mg | £449.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Typical lead time: 14-21 working days.
Contact us for more accurate information.
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Further Information
Additional Names:
ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL; IHC-P: 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
Background References:
- Masumoto et al. J Biol Chem. 1999;274(48):33835-8.
- Masumoto et al. Exp Cell Res. 2001;262(2):128-133.
- Conway et al. Cancer Res. 2000;60(22):6236-42
- McConnell et al. Cancer Res. 2000;60(22):6243-7. (WD0102)
Buffer:
ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse (57%), Rat (57%)
Immunogen:
Anti-ASC antibody (2287) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC.
The immunogen is located within the last 50 amino acids of ASC.
The immunogen is located within the last 50 amino acids of ASC.
ISOFORMS:
Human ASC has 3 isoforms, including isoform 1 (195aa, 22kD), isoform 2 (176aa, 20kD), and isoform 3 (135aa, 15kD). Mouse ASC has one isoform (193aa, 22kD) and rat ASC also has one isoform (193aa, 22kD). 2287 can detect all human isoforms.
NCBI Gene ID #:
29108
NCBI Official Name:
PYD and CARD domain containing
NCBI Official Symbol:
PYCARD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22kD
Observed: 22 kD
Observed: 22 kD
Protein Accession #:
BAA87339
Protein GI Number:
10801602
Purification:
ASC Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q9ULZ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Independent Antibody Validation in Cell lines (Figure 2) shows similar ASC expression profile in human and mouse cell lines detected by two independent anti-ASC antibodies that recognize different epitopes, 2287 against C-terminus domain and 39-001 against full length human recombinant protein. ASC proteins are detected in some of tested cell lines at different expression levels by the two independent antibodies.
Documents
References
- Dowds et al. Cryopyrin-induced interleukin 1beta secretion in monocytic cells: enhanced activity of disease-associated mutants and requirement for ASC. J Biol Chem. 2004;279(21):21924-8. PMID: 15020601
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