Further Information
Caspase-4 Antibody: TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, ICH2, Caspase-4, Protease ICH-2, CASP-4
Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.
- Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
- Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
- Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
- Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 β-converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.
Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (73%)
Caspase-4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Caspase-4.
The immunogen is located within amino acids 100 - 150 of Caspase-4.
837
caspase 4, apoptosis-related cysteine peptidase
CASP4
Homo sapiens
Liquid
AAA86890
886050
Caspase-4 Antibody is affinity chromatography purified via peptide column.
Apoptosis,Cancer,Neuroscience
Depending on cell lines or tissues used, other cleavage products may be observed.
P49662
Optimal dilutions for each application to be determined by the researcher.