ProSci

StrepII-tag Antibody

Product Code:
 
PSI-4217
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 1
Western blot analysis of 50 ng of StrepII-tagged recombinant protein with StrepII antibody at 0.25 μg/mL.
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Western blot analysis of 50 ng of StrepII-tagged recombinant protein with StrepII antibody at 0.25 μg/mL.

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CodeSizePrice
PSI-4217-0.02mg0.02mg£150.00
Quantity:
PSI-4217-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
StrepII-tag Antibody:
Application Note:
StrepII-tag antibody can be used for the detection of StrepII by Western blot at 0.25 μg/mL.
Background:
StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
Background References:
  • Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
  • Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.
Buffer:
StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
StrepII-tag antibody was raised against the amino acid sequence "gggWSHPQFEK".
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 100 kDa

Observed: 95 kDa
Purification:
StrepII-tag Antibody is affinity chromatography purified via peptide column.
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.