ProSci

ZBED1 Antibody

Product Code:
 
PSI-5111
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 1
Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.
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Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.

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CodeSizePrice
PSI-5111-0.02mg0.02mg£150.00
Quantity:
PSI-5111-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
ZBED1 Antibody: ALTE, DREF, TRAMP, hDREF, ALTE, KIAA0785, Zinc finger BED domain-containing protein 1, Putative Ac-like transposable element
Application Note:
ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
Background References:
  • Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38.
  • Yamashita D, Sano Y, Adachi Y, et al. hDREF regulates cell proliferation and expression of ribosomal protein genes. Mol. Cell. Biol.2007; 27:2003-13.
  • Yamashita D, Komori H, Higuchi Y, et al. Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding. J. Biol. Chem.2007; 282:7563-75.
Buffer:
ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1.

The immunogen is located within amino acids 180 - 230 of ZBED1.
NCBI Gene ID #:
9189
NCBI Official Name:
zinc finger, BED-type containing 1
NCBI Official Symbol:
ZBED1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH15030
Protein GI Number:
15929151
Purification:
ZBED1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
Swissprot #:
O96006
User NOte:
Optimal dilutions for each application to be determined by the researcher.