ProSci

PPAPDC3 Antibody

Product Code:
 
PSI-5519
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.
❮❯

Western blot analysis of PPAPDC3 in mouse heart tissue lysate with PPAPDC3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of PPAPDC3 in mouse heart tissue with PPAPDC3 antibody at 20 μg/mL.

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CodeSizePrice
PSI-5519-0.02mg0.02mg£150.00
Quantity:
PSI-5519-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
PPAPDC3 Antibody: NET39, C9orf67, Probable lipid phosphate phosphatase PPAPDC3, Phosphatidic acid phosphatase type 2 domain-containing protein 3
Application Note:
PPAPDC3 antibody can be used for detection of PPAPDC3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
PPAPDC3 Antibody: PPAPDC3, also known as nuclear envelope transmembrane protein 39 (NET39), was initially discovered in an in silico screen for secreted or membrane proteins. It is a member of the PAP2 superfamily of phosphatases and haloperoxidases. PPAPDC3 has recently been shown to act as a negative regulator of myoblast differentiation by diminishing the activity of the mammalian target of rapamycin TOR. PPAPDC3 is highly expressed in cardiac and skeletal muscle and becomes strongly upregulated during cultured myoblast differentiation tissues. Overexpression of PPAPDC3 in myoblasts repressed myogenesis while knockdown by RNA interference promoted differentiation indicating its part in the regulatory mechanism for myogenesis.
Background References:
  • Otsuki T, Ota T, Nishikawa T, et al. Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. DNA Res.2005; 12:117-26.
  • Liu GH, Guan T, Datta K, et al. Regulation of myoblast differentiation by the nuclear envelope protein NET39. Mol. Cell Biol.2009; 29:5800-12.
Buffer:
PPAPDC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PPAPDC3 antibody was raised against a 19 amino acid synthetic peptide from near the amino of terminus human PPAPDC3.

The immunogen is located within the first 50 amino acids of PPAPDC3.
NCBI Gene ID #:
84814
NCBI Official Name:
phosphatidic acid phosphatase type 2 domain containing 3
NCBI Official Symbol:
PPAPDC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW87966
Protein GI Number:
119608372
Purification:
PPAPDC3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q8NBV4
User NOte:
Optimal dilutions for each application to be determined by the researcher.