ProSci

Nucleobindin-2 Antibody

Product Code:
 
PSI-7271
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.
3 / 3
Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.
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Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.
Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.

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CodeSizePrice
PSI-7271-0.02mg0.02mg£150.00
Quantity:
PSI-7271-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Nucleobindin-2 Antibody: NEFA, HEL-S-109, NEFA, Nucleobindin-2, DNA-binding protein NEFA
Application Note:
Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
Background References:
  • Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85.
  • Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443:709-12.
  • Garcia-Galiano D, Navarro VM, Gaytan F, et al. Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J. Mol. Endocrin. 2010; 45:281-90.
Buffer:
Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 .

The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.
NCBI Gene ID #:
4925
NCBI Official Name:
nucleobindin 2
NCBI Official Symbol:
NUCB2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 54 kDa
Protein Accession #:
NP_005004
Protein GI Number:
4826870
Purification:
Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.
Swissprot #:
P80303
User NOte:
Optimal dilutions for each application to be determined by the researcher.