ProSci

SHISA4 Antibody

Product Code:
 
PSI-7277
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.
3 / 3
Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.
❮❯

Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.
Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.

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CodeSizePrice
PSI-7277-0.02mg0.02mg£150.00
Quantity:
PSI-7277-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
SHISA4 Antibody: TMEM58, C1orf40, TMEM58, UNQ583/PRO1153, Protein shisa-4, Transmembrane protein 58
Application Note:
SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SHISA4 Antibody: SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
Background References:
  • Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92.
  • Pei J and Grishin NV. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell Signal. 2012; 24:758-69.
  • Yamamoto A, Nagano T, Takehara S, et al. Shisa promotes head formation through the inhibition of receptor protein maturation for the caudalizing factors, Wnt and FGF. Cell 2005; 120:223-35.
Buffer:
SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4 .

The immunogen is located within amino acids 100 - 150 of SHISA4.
NCBI Gene ID #:
149345
NCBI Official Name:
shisa homolog 4 (Xenopus laevis)
NCBI Official Symbol:
SHISA4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22 kDa

Observed: 24 kDa
Protein Accession #:
NP_937792
Protein GI Number:
269973884
Purification:
SHISA4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
SHISA4 antibody is predicted to not cross-react with other SHISA protein family members.
Swissprot #:
Q96DD7
User NOte:
Optimal dilutions for each application to be determined by the researcher.