ProSci

MTERFD1 Antibody

Product Code:
 
PSI-7281
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 3
Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.
❮❯

Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.
Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-7281-0.02mg0.02mg£150.00
Quantity:
PSI-7281-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
MTERFD1 Antibody: CGI-12, mTERF3, mTERF domain-containing protein 1, mitochondrial, Mitochondrial transcription termination factor 3
Application Note:
MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
Background References:
  • Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
  • Hyvarinen AK, Pohjoismaki JL, Holt IJ, et al. Overexpression of MTERFD1 or MTERFD3 impairs the completion of mitochondrial DNA replication. Mol. Biol. Rep. 2011; 38:1321-8.
  • Prieto-Martin A, Montoya J, Martinez-Azorin F, et al. Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Res. 2004; 32:2059-68.
  • Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.
Buffer:
MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (88%), Chicken: (88%)
Immunogen:
MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1 .

The immunogen is located within the last 50 amino acids of MTERFD1.
NCBI Gene ID #:
51001
NCBI Official Name:
MTERF domain containing 1
NCBI Official Symbol:
MTERFD1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 42 kDa
Protein Accession #:
NP_057026
Protein GI Number:
34147676
Purification:
MTERFD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.
Swissprot #:
Q96E29
User NOte:
Optimal dilutions for each application to be determined by the researcher.