ProSci

Pescadillo Antibody

Product Code:
 
PSI-45-109
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-109 (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-109 (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-109-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PES1, pescadillo homolog 1, containing BRCT domain (zebrafish), PES, pescadillo (zebrafish) homolog 1, containing BRCT domain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in HeLa lysate (calculated MW of 68kDa according to NP_055118). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8ug/ml.
Background References:
  • Allende ML, Amsterdam A, Becker T, Kawakami K, Gaiano N, Hopkins N. Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev. 1996 Dec 15;10(24):3141-55.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Pig, Cow
Immunogen:
The immunogen for this antibody is: GGLEKKKYERGSA-C
NCBI Gene ID #:
23481
NCBI Official Name:
pescadillo ribosomal biogenesis factor 1
NCBI Official Symbol:
PES1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055118.1
Protein GI Number:
7657455
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
O00541