ProSci

APRIL (Sacha-2) Monoclonal Antibody

Product Code:
 
PSI-XA-1025
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rat
Antibody Isotype:
 
Rat IgG2a
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
Storage:
 
APRIL antibody can be stored at 4˚C, stable for one year.
 

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CodeSizePrice
PSI-XA-1025-0.1mg0.1mg£686.00
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Further Information

Application Note:
APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μg/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T? cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of Sacha-2 antibody.? After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).
NCBI Gene ID #:
8741 / 9370
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 13 / adiponectin, C1Q and collagen domain containing
NCBI Official Symbol:
TNFSF13 / ADIPOQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.
Research Area:
Cytokine,Cancer
Swissprot #:
O75888?/ Q15848
User NOte:
Optimal dilutions for each application to be determined by the researcher.