ProSci

ApoM Monoclonal Antibody

Product Code:
 
PSI-32-112
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1 & IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8F12C6B8
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
ApoM monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis using ApoM monoclonal antibody against human serum.
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Western blot analysis using ApoM monoclonal antibody against human serum.

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PSI-32-112-0.1mL0.1mL£626.00
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Further Information

Additional Names:
G3A, NG20, HSPC336, Protein G3a, Apo-M, G3a, apo-M
Application Note:
Western Blot:1:500 - 1:2,000
IF:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21 kDa),is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.
Background References:
  • Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286.
  • Duan J, Dahlback B, Villoutreix BO.FEBS Lett. 2001 Jun 15;499(1-2):127-32.
  • Xu,N., Nilsson-Ehle,P. & Ahren,B. 2004. J. Nutr.Biochem. 15 (10):579-582.
  • Zhang,X.Y. ,et al.2004. Acta Histochem. 106 (2):123-128
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
55937
NCBI Official Name:
apolipoprotein M
NCBI Official Symbol:
APOM
NCBI Organism:
Homo sapiens
Protein Accession #:
O95445
Protein GI Number:
17370872
Purification:
Antibody is purified by protein A affinity chromatography.
Swissprot #:
O95445
User NOte:
Optimal dilutions for each application to be determined by the researcher.