ProSci

VPREB3 Antibody

Product Code:
 
PSI-42-296
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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CodeSizePrice
PSI-42-296-0.1mg0.1mg£505.00
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Further Information

Additional Names:
VPREB3, pre-B lymphocyte 3, 8HS20, N27C7-2, pre-B lymphocyte gene 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 26kDa band in Human Lymph Node lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.7kDa according to NP_037510.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Rosnet O, Blanco-Betancourt C, Grivel K, Richter K, Schiff C, Binding of free immunoglobulin light chains to VpreB3 inhibits their maturation and secretion in chicken B cells. The Journal of biological chemistry 2004 Mar 279 (11): 10228-36.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: EAHNACVLTISPVQ
NCBI Gene ID #:
29802
NCBI Official Name:
pre-B lymphocyte 3
NCBI Official Symbol:
VPREB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037510.1
Protein GI Number:
7019567
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell ,Immunology
Swissprot #:
Q9UKI3