ProSci

eNOS Antibody

Product Code:
 
PSI-79-565
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).
2 / 2
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-1177).
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Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-1177).

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PSI-79-565-0.1mg0.1mg£481.00
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Further Information

Additional Names:
eNOS, ECNOS, Constitutive NOS, EC-NOS, NOS3, NOSIII
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Background References:
  • Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M. B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S. R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
eNOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
NCBI Gene ID #:
4846
NCBI Official Name:
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol:
NOS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
140 kDa
Protein Accession #:
NP_000594.2
Protein GI Number:
40254422
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total eNOS protein.
Swissprot #:
P29474