ProSci

MIP-1a Antibody

Product Code:
 
PSI-38-220
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
MIP-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
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To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MIP-1-alpha (38-222) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-alpha.
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To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MIP-1-alpha (38-222) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-alpha.

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CodeSizePrice
PSI-38-220-0.1mg0.1mg£524.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha
Application Note:
ELISA:
Indirect:
To detect hMIP-1a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.

Sandwich
To detect hMIP-1a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human MIP-1a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.

Western Blot:
To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Background:
MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (human MIP-1 alpha). Human MIP-1a specific antibody was purified by affinity chromatography employing immobilized hMIP-1a matrix.
NCBI Gene ID #:
6348
NCBI Official Name:
chemokine (C-C motif) ligand 3
NCBI Official Symbol:
CCL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10147
Protein GI Number:
127078
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P10147
User NOte:
Centrifuge vial prior to opening.