ProSci

Tryptophan Hydroxylase Antibody

Product Code:
 
PSI-50-242
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Amphibian
  • Bovine (Cattle)
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
Tryptophan Hydroxylase antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
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Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.
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Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

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PSI-50-242-0.1mL0.1mL£686.00
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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et
Background References:
  • Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017.
  • Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823.
  • Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2).
Modifications:
Phospho-Specific
NCBI Gene ID #:
317675
NCBI Official Name:
tryptophan hydroxylase 2
NCBI Official Symbol:
Tph2
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55
Protein Accession #:
Q8CGU9
Protein GI Number:
30580595
Purification:
Affinity Purified
Research Area:
Phospho-Specific ,Neuroscience
Swissprot #:
Q8CGU9
User NOte:
Optimal dilutions for each application to be determined by the researcher.