Soluble RANK Ligand Recombinant Protein

ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate na?ve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant human sRANKL is a 20.0 kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).

Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. The expected ED50 for this effect is 10.0-25.0 ng/ml.

8600

tumor necrosis factor (ligand) superfamily, member 11

TNFSF11

Homo sapiens

Lyophilized

NP_003692.1

4507595

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

#N/A

E. coli

O14788