ProSci

Unconjugated Goat Anti-Rabbit IgG (H&L) Antibody Pre-Adsorbed

Product Code:
 
PSI-XR-9900
Product Group:
 
Secondary Antibody
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rabbit
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Unconjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear a
 

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PSI-XR-9900-2mg2mg£404.00
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Further Information

Application Note:
Unconjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry. Recommended dilutions are as follows, ELISA 1:35,000 - 1:325,000, Western Blot 1:3,000 - 1:35,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user).
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2
Concentration:
2.0 mg/mL
Conjugate:
Unconjugated
Immunogen:
Rabbit IgG - whole molecule
Physical State:
Liquid
Purification:
Unconjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.?? Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.? No reaction was observed against Human Serum Proteins.? Specificity was confirmed by ELISA at less than 0.5%

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