Further Information
Lnhr, CD62L, Ly-22, Lyam1, Ly-m22, Lyam-1, LECAM-1, AI528707, L-selectin, Sell
The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin: 74 kDa (on lymphocytes) or 95 kDa (on neutrophils).In the Mouseorganism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes.
- Gallatin, W. M., Weissman, I. L., Butcher, E. C. (1982). A cell-surface molecule involved in organ-specific homing of lymphocytes.Nature,304(5921), 30-34.
- Siegelman, M. H., Cheng, I. C., Weissman, I. L., Wakeland, E. K. (1990). The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.Cell,61(4), 611-622.
- Pizcueta, P., Luscinskas, F. W. (1994). Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo.The American journal of pathology,145(2), 461.
Phosphate-buffered aqueous solution, ?0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
20343
selectin, lymphocyte
Sell
Mus musculus
liquid
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Immunology,Functional Assays
The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains.
P18337
Optimal dilutions for each application to be determined by the researcher.