ProSci

Rspondin 1 Recombinant Protein

Product Code:
 
PSI-96-658
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-658-0.05mg0.05mg£765.00
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Further Information

Additional Names:
RSPO1, CRISTIN3, FLJ40906, RP11-566C13.1, R-spondin-1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
Background References:
  • Chen, JZ. et al. ,2002, Mol Biol Rep. 2002;29:287.
  • Kim, K.A. et al., 2006, Cell Cycle. 2006;5:23?26.
  • Nam, J.S. et al., 2007, Gene Expr. Patterns 7, 306?312.
  • Kazanskaya, O. et al., 2004, Dev. Cell 7:525.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20μg/ml (100μl/well) can bind human LIMPII with a linear ranger of 31. 25 - 1000 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
284654
NCBI Official Name:
R-spondin homolog (Xenopus laevis)
NCBI Official Symbol:
RSPO1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
28.7 kDa
Protein Accession #:
NP_001033722.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q2MKA7

Documents