ProSci

PNP Antibody

Product Code:
 
PSI-43-273
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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43-273 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-273 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-43-273-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
PNP, purine nucleoside phosphorylase, NP, PRO1837, PUNP, inosine phosphorylase, purine-nucleoside:orthophosphate ribosyltransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of Human Liver, Erythrocytes and of cell lines Daudi, Jurkat and K562 (calculated MW of 32.1kDa according to NP_000261.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Vareed SK, et al.Metabolites of purine nucleoside phosphorylase (NP) in serum have the potential to delineate pancreatic adenocarcinoma.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SGQNPLRGPNDE
NCBI Gene ID #:
4860
NCBI Official Name:
purine nucleoside phosphorylase
NCBI Official Symbol:
PNP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_000261.2
Protein GI Number:
157168362
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Obesity,Signal Transduction,
Swissprot #:
P00491