TDO Anopheles gambiae Recombinant Protein

TPH2, TRPO, Tryptophan 2,3-dioxygenase, Tryptophan Oxygenase, Tryptophan Pyrrolase, Tryptophanase

This recombinant proteins is for research use only.

Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.

Specific activity towards L-Trp: 87.0umol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).

In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol.

0.5mg/ml (Lot specific)

His Tag

1273245

AGAP002721-PA

T23O_ANOGA

Anopheles gambiae str. PEST

Liquid

O77457

74764641

>98% (SDS-PAGE)

Thr32-Gly402

E. coli

Q01151