NAD Synthetase B. subtilis Recombinant Protein

NH(3)-dependent NAD(+) Synthetase, General Stress Protein 38, GSP38, Spore Outgrowth Factor B, EC6.3.1.5

This recombinant proteins is for research use only.

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+

~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol NAD per min.

In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT.

0.5mg/ml (Lot specific)

Tag Free

Liquid

P08164

>98% (SDS-PAGE)

Thr32-Gly402

E. coli

P16410