ProSci

LIF Recombinant Protein

Product Code:
 
PSI-91-014
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Storage:
 
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
 

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CodeSizePrice
PSI-91-014-0.05mg0.05mg£657.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, HILDA
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Biological Activity:
ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.
Specific Activity of 1.0 x 108 IU/mg.
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
Tag Free
NCBI Gene ID #:
3976
NCBI Official Name:
leukemia inhibitory factor
NCBI Official Symbol:
LIF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
19.7 kD
Protein Accession #:
P15018
Protein GI Number:
20149322
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P15018

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