Chemodex

Biotin-(5-fluorescein)-conjugate

Product Code:
 
CDX-B0078
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
Short Term: +4°C. Long Term: -20°C
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Chemical Structure
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Chemical Structure

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CDX-B0078-M0011 mg£84.00
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CDX-B0078-M0055 mg£304.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
Biotin-4-Fluorescein; B4F; N-(Biotinylamidoethyl)-fluorescein-5-carboxamide; N-(Fluorescein-5-carbamidoethyl)biotinamide
Appearance:
Yellow solid.
CAS:
957494-27-8
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1
InChiKey:
DFUFXKZUEOKPSD-LFERIPGTSA-N
Long Description:
Chemical. CAS: 957494-27-8. Formula: C33H32N4O8S. MW: 644.7. Synthetic. Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
MDL:
MFCD03791084
Molecular Formula:
C33H32N4O8S
Molecular Weight:
644.7
Package Type:
Vial
Product Description:
Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
Purity:
>90% (HPLC)
SMILES:
O=C1C=C2OC3=CC(O)=CC=C3C(C4=C(C(O)=O)C=C(C(NCCNC(CCCC[C@H]5[C@](NC(N6)=O)([H])[C@]6([H])CS5)=O)=O)C=C4)=C2C=C1
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

(1) G. Kada et al. Biochim. Biophys. Acta 1427, 33 (1999) | (2) G. Kada et al. Biochim. Biophys. Acta 1427, 44 (1999) | (3) N. Humbert, et al.; Electrophoresis 26, 47 (2005) | (4) F.M. Aslan, et al.; PNAS 102, 8507 (2005) | (5) M.J. Waner & D.P. Mascotti; J. Biochem. Biophys. Methods 70, 873 (2008) | (6) A. Ebner, et al.; Methods Mol. Biol. 418, 73 (2008) | (7) R. Mittal & M.P. Bruchez; Bioconjug. Chem. 22, 362 (2011)