ProSci

ENPP7 Antibody

Product Code:
 
PSI-61-716
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of ENPP7 Antibody in HL-60 cell line lysates (35ug/lane)
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ENPP7 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-716-400ul400ul£626.00
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Further Information

Additional Names:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase, ENPP7 {ECO:0000312|EMBL:AAH414532}
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ENPP7 converts sphingomyelin to ceramide. ENPP7 also has phospholipase C activity toward palmitoyl lyso-phosphocholine. ENPP7 does not appear to have nucleotide pyrophosphatase activity.
Background References:
  • Wu, J., et al. Biochem. J. 386 (PT 1), 153-160 (2005)
  • Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
  • Wu, J., et al. Carcinogenesis 25(8):1327-1333(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ENPP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-313 amino acids from the Central region of human ENPP7.
NCBI Gene ID #:
339221
NCBI Official Name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
NCBI Official Symbol:
ENPP7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q6UWV6
Protein GI Number:
134047772
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q6UWV6
User NOte:
Optimal dilutions for each application to be determined by the researcher.