ProSci

CCNB1 Antibody

Product Code:
 
PSI-55-622
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-622-400ul400ul£626.00
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Further Information

Additional Names:
G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].
Background References:
  • Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010)
  • van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010)
  • Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010)
  • Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Zebrafish, Hamster, B
Immunogen:
This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-268 amino acids from the Central region of human CCNB1.
NCBI Gene ID #:
891
NCBI Official Name:
G2/mitotic-specific cyclin-B1
NCBI Official Symbol:
CCNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P14635
Protein GI Number:
116176
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P14635
User NOte:
Optimal dilutions for each application to be determined by the researcher.