Further Information
Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL
(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.
The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of
NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,
MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by
trapping it in the cytoplasm. Phosphorylation of serine residues on
the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM
603258) marks them for destruction via the ubiquitination pathway,
thereby allowing activation of the NFKB complex. Activated NFKB
complex translocates into the nucleus and binds DNA at
kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
- Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010)
- Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010)
- Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010)
- Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Pig, Mouse
This NFKBp65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-539 amino acids from the C-terminal region of human NFKBp65.
5970
Transcription factor p65
RELA
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Q04206
62906901
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle,Immunology,Obesity,Neuroscience,Signal Transduction
Q04206
Optimal dilutions for each application to be determined by the researcher.