Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western blot analysis of AKT2 using rabbit polyclonal AKT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2). Western blot analysis of AKT2 using rabbit polyclonal AKT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).](https://www.prosci-inc.com/static-images/62-605_1.jpg)
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![Western blot analysis in 293 cell line lysates (35ug/lane). Western blot analysis in 293 cell line lysates (35ug/lane).](https://www.prosci-inc.com/static-images/62-605_2.jpg)
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![](https://www.prosci-inc.com/static-images/62-605_3.jpg)
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![Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/62-605_4.jpg)
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![AKT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. AKT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.](https://www.prosci-inc.com/static-images/62-605_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
- Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003).
- Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003).
- Jones, D.T., et al., Blood 101(8):3174-3180 (2003).
- Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human AKT2.
208
RAC-beta serine/threonine-protein kinase
AKT2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
P31751
1170703
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle,Immunology,Obesity,Neuroscience,Signal Transduction
P31751
Optimal dilutions for each application to be determined by the researcher.