ProSci

Myeloperoxidase Antibody

Product Code:
 
PSI-57-644
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).
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Myeloperoxidase Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Myeloperoxidase Antibody for immunohistochemistry.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the detected the Myeloperoxidase protein (arrow).
Myeloperoxidase Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Myeloperoxidase Antibody for immunohistochemistry.

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PSI-57-644-400ul400ul£626.00
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Further Information

Additional Names:
Myeloperoxidase, MPO, Myeloperoxidase, 89 kDa myeloperoxidase, 84 kDa myeloperoxidase, Myeloperoxidase light chain, Myeloperoxidase heavy chain, MPO
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq].
Background References:
  • Banerjee, M., et al. Toxicol. Appl. Pharmacol. 249(1):47-54(2010)
  • Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
  • Nahon, P., et al. Antioxid. Redox Signal. (2010) In press :
  • Wang, Y., et al. J. Huazhong Univ. Sci. Technol. Med. Sci. 30(4):437-442(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Myeloperoxidase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-448 amino acids from the Central region of human Myeloperoxidase.
NCBI Gene ID #:
4353
NCBI Official Name:
Myeloperoxidase
NCBI Official Symbol:
MPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
P05164
Protein GI Number:
129825
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Obesity
Swissprot #:
P05164
User NOte:
Optimal dilutions for each application to be determined by the researcher.