ProSci

BCDIN3D Antibody

Product Code:
 
PSI-61-351
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-61-351-200ul200ul£626.00
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Further Information

Additional Names:
Pre-miRNA 5'-monophosphate methyltransferase, 2.1.1.-, BCDIN3 domain-containing protein, BCDIN3D
Application Note:
For WB starting dilution is: 1:1000-2000
Background:
O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.
Background References:
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  • Xhemalce B.,et al.Cell 151:278-288(2012).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D.
NCBI Gene ID #:
144233
NCBI Official Name:
Pre-miRNA 5'-monophosphate methyltransferase
NCBI Official Symbol:
BCDIN3D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q7Z5W3
Protein GI Number:
74738762
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q7Z5W3
User NOte:
Optimal dilutions for each application to be determined by the researcher.