ProSci

PIK3R5 Antibody

Product Code:
 
PSI-55-906
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in WiDr cell line lysates (35ug/lane).
2 / 3
PIK3R5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in WiDr cell line lysates (35ug/lane).
PIK3R5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-906-400ul400ul£626.00
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Further Information

Additional Names:
Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit, PtdIns-3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K, PIK3R5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit (Brock et al., 2003 [PubMed 12507995]).
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Segat, L., et al. Vaccine 28(10):2201-2206(2010)
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
  • Johnson, C., et al. Oncogene 26(49):7049-7057(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIK3R5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-792 amino acids from the C-terminal region of human PIK3R5.
NCBI Gene ID #:
23533
NCBI Official Name:
Phosphoinositide 3-kinase regulatory subunit 5
NCBI Official Symbol:
PIK3R5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q8WYR1
Protein GI Number:
74716480
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8WYR1
User NOte:
Optimal dilutions for each application to be determined by the researcher.