ProSci

COL8A2 Antibody

Product Code:
 
PSI-56-083
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in CEM cell line lysates (35ug/lane).
2 / 3
COL8A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in CEM cell line lysates (35ug/lane).
COL8A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-083-400ul400ul£626.00
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Further Information

Additional Names:
Collagen alpha-2(VIII) chain, Endothelial collagen, COL8A2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.
Background References:
  • Hemadevi, B., et al. BMC Ophthalmol 10,(2010) :
  • Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
  • Liskova, P., et al. BrOphthalmol 91(12):1717-1718(2007)
  • Aldave, A.J., et al. Cornea 26(8):963-965(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
NCBI Gene ID #:
1296
NCBI Official Name:
Collagen alpha-2(VIII) chain
NCBI Official Symbol:
COL8A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
67 kDa
Protein Accession #:
P25067
Protein GI Number:
45644957
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P25067
User NOte:
Optimal dilutions for each application to be determined by the researcher.