ProSci

WNT1 Antibody

Product Code:
 
PSI-62-460
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of WNT1 Antibody in mouse heart tissue lysates (35ug/lane)
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WNT1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of WNT1 Antibody in mouse heart tissue lysates (35ug/lane)
WNT1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-460-400ul400ul£626.00
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Further Information

Additional Names:
Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, WNT1, INT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
WNT1 is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum.
Background References:
  • Shuai,X., et.al., Cancer Genet. Cytogenet. 194 (2), 119-124 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human WNT1.
NCBI Gene ID #:
7471
NCBI Official Name:
Proto-oncogene Wnt-1
NCBI Official Symbol:
WNT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
P04628
Protein GI Number:
139743
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P04628
User NOte:
Optimal dilutions for each application to be determined by the researcher.