ProSci

NSF Antibody

Product Code:
 
PSI-60-920
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).

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PSI-60-920-400ul400ul£626.00
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Further Information

Additional Names:
Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF
Application Note:
For WB starting dilution is: 1:1000
Background:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
Background References:
  • Saus, E., et al.Psychiatr Res 44(14):971-978(2010)
  • Hamza, T.H., et al. Nat. Genet. 42(9):781-785(2010)
  • Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
  • Parashuraman, S., et al. FEBS Lett. 584(6):1251-1256(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Rat
Immunogen:
This NSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-696 amino acids from the C-terminal region of human NSF.
NCBI Gene ID #:
4905
NCBI Official Name:
Vesicle-fusing ATPase
NCBI Official Symbol:
NSF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
P46459
Protein GI Number:
257051048
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P46459
User NOte:
Optimal dilutions for each application to be determined by the researcher.