NSJ Bioreagents

Lipocalin 2 Antibody / NGAL / LCN2

Product Code:
 
NSJ-R31946
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Lipocalin 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of 1) rat spleen and 2) rat lung lysate with Lipocalin 2 antibody. Predicted/observed molecular weight: 22-25 kDa.
2 / 7
IHC testing of FFPE mouse spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 7
IHC testing of FFPE rat spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
IHC testing of frozen mouse spleen tissue with Lipocalin 2 antibody.
5 / 7
IHC testing of frozen mouse lung tissue with Lipocalin 2 antibody.
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IHC testing of frozen rat spleen tissue with Lipocalin 2 antibody.
7 / 7
IHC testing of frozen rat lung tissue with Lipocalin 2 antibody.
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Western blot testing of 1) rat spleen and 2) rat lung lysate with Lipocalin 2 antibody. Predicted/observed molecular weight: 22-25 kDa.
IHC testing of FFPE mouse spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen mouse spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen mouse lung tissue with Lipocalin 2 antibody.
IHC testing of frozen rat spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen rat lung tissue with Lipocalin 2 antibody.

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NSJ-R31946-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
Optimal dilution of the Lipocalin 2 antibody should be determined by the researcher.
Description:
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 21-200 of mouse Lipocalin 2 were used as the immunogen for the Lipocalin 2 antibody.
Limitation:
This Lipocalin 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P11672