NSJ Bioreagents

MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)

Product Code:
 
NSJ-R30048
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MAPK-YT, aka IL-13
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
  • Yeast
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
 

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CodeSizePrice
NSJ-R30048-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: Suitable
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
5594
Immunogen:
A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH was used as the immunogen for this MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2).
Limitation:
This MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat, yeast