NSJ Bioreagents

PIAS1 Antibody

Product Code:
 
NSJ-R31376
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PIAS1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 6
Western blot testing of PIAS1 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) Jurkat; 5: (h) MCF-7; 6: (h) SKOV lysate; Expected size: 72~100KD
2 / 6
IHC-P: PIAS1 antibody testing of human placenta tissue
3 / 6
ICC testing of PIAS1 antibody and A549 cells
4 / 6
IHC-P: PIAS1 antibody testing of rat testis tissue
5 / 6
IHC-P: PIAS1 antibody testing of rat brain tissue
6 / 6
❮❯

Western blot testing of PIAS1 antibody and Lane 1: rat testis; 2: mouse testis; 3: human HeLa; 4: (h) Jurkat; 5: (h) MCF-7; 6: (h) SKOV lysate; Expected size: 72~100KD
IHC-P: PIAS1 antibody testing of human placenta tissue
ICC testing of PIAS1 antibody and A549 cells
IHC-P: PIAS1 antibody testing of rat testis tissue
IHC-P: PIAS1 antibody testing of rat brain tissue

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R31376-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat).
Limitation:
This PIAS1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75925