NSJ Bioreagents

RFC1 Antibody

Product Code:
 
NSJ-R30806
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RFC1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of RFC1 antibody and human samples: 1) HeLa, 2) SMMC-7721, 3) Jurkat, 4) CCRF-CEM, 5) 293T cell lysate.~
2 / 2
IHC testing of FFPE human breast cancer tissue with RFC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate
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Western blot testing of RFC1 antibody and human samples: 1) HeLa, 2) SMMC-7721, 3) Jurkat, 4) CCRF-CEM, 5) 293T cell lysate.~
IHC testing of FFPE human breast cancer tissue with RFC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate

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CodeSizePrice
NSJ-R30806-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the RFC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
Limitation:
This RFC1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35251