NSJ Bioreagents

RIAM Antibody

Product Code:
 
NSJ-R31063
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RIAM antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of RIAM antibody and human 1) ThP-1, 2) Jurkat, 3) Raji, 4) U937, 5) rat thymus and 6) mouse thymus lysate. Predicted molecular weight ~73~110 kDa.
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IHC-P: RIAM antibody testing of human tonsil tissue
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ICC testing of RIAM antibody and Jurkat cells
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IHC-P: RIAM antibody testing of rat lymph tissue
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IHC-F: RIAM antibody testing of rat spleen tissue
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Immunofluorescent staining of FFPE human A431 cells with RIAM antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human SiHa cells with RIAM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIAM antibody.
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Western blot testing of RIAM antibody and human 1) ThP-1, 2) Jurkat, 3) Raji, 4) U937, 5) rat thymus and 6) mouse thymus lysate. Predicted molecular weight ~73~110 kDa.
IHC-P: RIAM antibody testing of human tonsil tissue
ICC testing of RIAM antibody and Jurkat cells
IHC-P: RIAM antibody testing of rat lymph tissue
IHC-F: RIAM antibody testing of rat spleen tissue
Immunofluorescent staining of FFPE human A431 cells with RIAM antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with RIAM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIAM antibody.

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CodeSizePrice
NSJ-R31063-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the RIAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
RIAM (Rap1-interacting adaptor molecule), also called RARP1 and APBB1-Interacting Protein, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al.(2004) mapped the gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al.(2003) found that RIAM suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RIAM suppressed AP1 transactivation. Lafuente et al.(2004) found that the protein interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Rap1-interacting adaptor molecule (EQDFMSDLMKALQKKRGNVS) was used as the immunogen for this RIAM antibody.
Limitation:
This RIAM antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
Q7Z5R6