NSJ Bioreagents

RIG-I Antibody (DDX58)

Product Code:
 
NSJ-R32826
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the RIG-I antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus, 5) mouse NIH3T3 and 6) human Jurkat lysate with RIG-I antibody at 0.5ug/ml. Expected molecular weight: 106-115 kDa.
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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus, 5) mouse NIH3T3 and 6) human Jurkat lysate with RIG-I antibody at 0.5ug/ml. Expected molecular weight: 106-115 kDa.

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NSJ-R32826-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the RIG-I antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
RIG-I (retinoic acid-inducible gene I) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded (in humans) by the DDX58 gene. RIG-I is part of the RIG-I-like receptor family, which also includes MDA5 and LGP2, and functions as a pattern recognition receptor that is a sensor for viruses such as influenza A, Sendai virus, and flavivirus. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids H871-K925 was used as the immunogen for the RIG-I antibody.
Limitation:
This RIG-I antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95786