NSJ Bioreagents

RNF43 Antibody

Product Code:
 
NSJ-RQ4411
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RNF43 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of human 1) HeLa, 2) 293T, 3) COLO-320, 4) SW620 and 5) MCF7 cell lysate with RNF43 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa (isoform 1), ~95 kDa (isoform 4).
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Western blot testing of human 1) HeLa, 2) 293T, 3) COLO-320, 4) SW620 and 5) MCF7 cell lysate with RNF43 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa (isoform 1), ~95 kDa (isoform 4).

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NSJ-RQ4411-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein) (BSA-free format available)
Application Note:
Optimal dilution of the RNF43 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human partial recombinant protein corresponding to amino acids G24-Y197 was used as the immunogen for the RNF43 antibody.
Limitation:
This RNF43 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q68DV7