Welcome to An Der Grub - distributed exclusively in the UK by Caltag Medsystems

Intracellular Flow Cytometry Reagents

An Der Grub Bio Research GmbH based in Vienna, Austria is recognized worldwide as a leading expert in the field of intracellular flow cytometry. Through their pioneering efforts An Der Grub designed and developed the pre-eminent reagent in cell fixation and permeabilization (FIX&PERM®).

FIX&PERM® allows for mild fixation and permeabilization of cells while leaving their morphological scatter characteristics intact enabling today's researcher to accurately identify previously undetectable intracellular markers.

Together with FIX&PERM®, An Der Grub also offer a selective panel of antibody conjugates for the analysis of intracellular human leukocyte antigens (COMBI-IC), SINGLE-IC) and selected antibodies for studies of human leukocyte surface molecules (SURFACE Antibodies). Plus, ADG-Lyse; an ideal reagent for whole blood staining of human surface antigens.

An Der Grub reagents continue to benchmark the field with high quality and consistent products, which are used in leading laboratories worldwide.

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FLOW CYTOMETRIC ANALYSIS OF INTRACELLULAR ANTIGENS

SINGLE-IC Antibodies/Conjugates
Specificity	Host	Clone	Isotype	Format	Product Code.	Application

CD3	Mouse	UCHT1	IgG1	Purified	GM-4011	Multigene receptor structure providing the signaling-sheath for the T cell antigen receptor (TCR). Identifies and enumerates immature and mature human T lymphocytes (T lineage marker).
				FITC	GM-4012
				PE	GM-4013
CD22	Mouse	RFB4	IgG1	Purifed	GM-4051	Identifies and enumerates B lymphocytes. Cytoplasmic expression in mature B cells, late pro- and early pre-B cells (B lineage marker).
				FITC	GM-4052
				PE	GM-4053
Lactoferrin	Mouse	aC5	IgG1	Purifed	GM-4111	Intracellular expression in specific granules. Identifies leukocytes with granulocytic differentiation, i.e. neutrophils.
				PE	GM-4113
Myeloperoxidase	Mouse	8E6	IgG1	Purified	GM-4191	Identifies cells with myeloid differentiation, with low expression levels in monocytes. Represents the most important myeloid lineage marker.
(MPO-C2)				FITC	GM-4192
				PE	GM-4193
Lysozyme	Mouse	LZ-2	IgG1	Purified	GM-4131	Identifies leukocytes with myeloid (monocytic) differentiation (monocyte lineage marker). Strongly positive in monocytes.
				FITC	GM-4132
				PE	GM-4133
CD68	Mouse	Ki-M7	IgG1	FITC	GM-4152	Identifies monocytes (strong cytoplasmic) and macrophages, also expressed in subsets of B cells.
IL-1ß	Mouse	FIB3	IgG1	FITC	GM-4162	Major effector cytokine of monocytes/macrophages, induced by e.g. bacterial products (LPS), for intracellular detection of IL-1β.
IFN-γ	Mouse	GZ4	IgG1	FITC	GM-4172	Major effector cytokine of T helper 1 CD4+ T cells, for intracellular detection of IFN-γ.

COMBI-IC Antibodies/Conjugates

Specificity	Host	Clone	Isotype	Format	Product Code.	Application

Negative Control	Mouse	VI-AP	IgG1	FITC	GIC-201	Non-binding isotype control antibody, for determination of non-specific binding of Ab via e.g. Fc-receptors to cell population of interest
	Mouse		IgG1	PE
Lysozyme	Mouse	LZ-2	IgG1	FITC	GIC-206	Combi-reagent differentiates between granulocytic (LZ+/LF+) and monocytic (LZ+/LF-) cell populations in e.g. bone marrow aspirates, peripheral blood.
Lactoferrin	Mouse	4C5	IgG1	PE
Myeloperoxidase (MPO-C2)	Mouse	8E6	IgG1	FITC	GIC-212	Combi-reagent differentiates between granulocytic (MPO+/LF+) and monocytic (MPO+/LF-) cell populations in e.g. bone marrow aspirates, peripheral blood.
Lactoferrin	Mouse	4C5	IgG1	PE
Myeloperoxidase (MPO-C2)	Mouse	8E6	IgG1	FITC	GIC-213	Combi-reagent differentiats between myeloid lineage (MPO+/CD3-) and T cell committed (MPO- /CD3+) cell populations in e.g. leukemia screening/typing of bone marrow aspirates, peripheral blood.
CD3	Mouse	UCHT1	IgG1	PE
Myeloperoxidase (MPO-C2)	Mouse	8E6	IgG1	FITC	GIC-214	Combi-reagent differentiates between myeloid lineage (MPO+/CD22-) and B cell committed (MPO- /CD22+) cell populations, e.g. in leukemia typing of bone marrow aspirates, peripheral blood
CD22	Mouse	RFB4	IgG1	PE

Single Surface Antibodies/Conjugates

Specificity	Host	Clone	Isotype	Format	Product Code.	Application

CD3	Mouse	UCHT1	IgG1	Purifed	GM-4011	Multigene surface receptor structure representing the signaling-sheath of the T cell antigen receptor (TCR). Identifies and enumerates mature human T lymphocytes (mature T lineage marker) and a subset of thymocytes
				FITC	GM-4012
				PE	GM-4013
CD22	Mouse	RFB4	IgG1	Purifed	GM-4051	Identifies and enumerates mature B lymphocytes. Surface-expressed on mature B-cells (B lineage marker)
				FITC	GM-4052
				PE	GM-4053
CD45	Mouse	VIT200	IgG1	Purifed	GM-4061	Pan-leukocyte marker molecule. Identifies hematopoietic cells, is down-regulated on distinct leukemia blasts, is therefore used as gating-marker for blasts in leukemia screening/typing.
				FITC	GM-4062
CD14	Mouse	MEM18	IgG1	Purifed	GM-4091	Monocyte differentiation antigen, highly expressed on mature monocytes (with low expression levels also on neutrophils) (monocyte lineage marker)
				FITC	GM-4092
				PE	GM-4093
CD65s	Mouse	VIM2	IgG1	Purifed	GM-4101	Fucogangliosid, identifies acute myeloid leukemia cells and distinct pre-B ALL cells. Also expressed on normal granulocytes and monocytes.
				FITC	GM-4102
CD117 (c-kit)	Mouse	95C3	IgG1	Purifed	GM-4141	Stem cell factor receptor, used to detect hematopoietic progenitor cells (most lineages) and mast cells.
				PE	GM-4143
TCR α/β	Mouse	BMA031	IgG1	PE	GM-4183	Pan T cell antigen receptor antibody, identifies mature T cells expressing the alpha/beta TCR (>90% of peripheral blood T cells).

Combi Surface Antibodies/Conjugates

Specificity	Host	Clone	Isotype	Format	Product Code.	Application

COMBI Gate Control					GCT-201	Combi-reagent identifies leukocytes (CD45+) and differentiates them from monocytes (CD14+) used as gating control to unequivocally identify major cellular populations (lymphocytes, monocytes, granulocytes) in whole blood, bone marrow etc.
CD45	Mouse	VIT200	IgG2a	FITC
CD14	Mouse	MEM18	IgG1	PE
COMBI Negative					GCT-202	Combi-reagent used as negative control to identify non-specific binding of antibodies to cell types of interest (e.g. via Fc-receptors).
Control	Mouse		IgG2a	FITC
	Mouse		IgG1	PE

Isotype Controls

Specificity	Host	Clone	Isotype	Format	Product Code.	Application

Negative Control	Mouse		IgG2a	FITC	GM-4982	Negative control to identify non-specific binding of respective antibody subclass to cell types of interest (e.g. via Fc-receptors).
Negative Control	Mouse		IgG2a	PE	GM-4983
Negative Control	Mouse		IgG1	Purified	GM-4991
Negative Control	Mouse		IgG2	FITC	GM-4992
Negative Control	Mouse		IgG3	PE	GM-4993

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