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Welcome to An Der Grub - distributed exclusively in the UK by Caltag Medsystems
Intracellular Flow Cytometry Reagents
An Der Grub Bio Research GmbH based in Vienna, Austria is recognized worldwide as a leading expert in the field of intracellular flow cytometry. Through their pioneering efforts An Der Grub designed and developed the pre-eminent reagent in cell fixation and permeabilization (FIX&PERM®).
FIX&PERM® allows for mild fixation and permeabilization of cells while leaving their morphological scatter characteristics intact enabling today's researcher to accurately identify previously undetectable intracellular markers.
Together with FIX&PERM®, An Der Grub also offer a selective panel of antibody conjugates for the analysis of intracellular human leukocyte antigens (COMBI-IC), SINGLE-IC) and selected antibodies for studies of human leukocyte surface molecules (SURFACE Antibodies). Plus, ADG-Lyse; an ideal reagent for whole blood staining of human surface antigens.
An Der Grub reagents continue to benchmark the field with high quality and consistent products, which are used in leading laboratories worldwide.
Information
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Products (click to view)
| FLOW CYTOMETRIC ANALYSIS OF INTRACELLULAR ANTIGENS | ||||||
| SINGLE-IC Antibodies/Conjugates | ||||||
| Specificity | Host | Clone | Isotype | Format | Product Code. | Application |
| CD3 | Mouse | UCHT1 | IgG1 | Purified | GM-4011 | Multigene receptor structure providing the signaling-sheath for the T cell antigen receptor (TCR). Identifies and enumerates immature and mature human T lymphocytes (T lineage marker). |
| FITC | GM-4012 | |||||
| PE | GM-4013 | |||||
| CD22 | Mouse | RFB4 | IgG1 | Purifed | GM-4051 |
Identifies and enumerates B lymphocytes. Cytoplasmic expression in mature B cells, late pro- and early pre-B cells (B lineage marker). |
| FITC | GM-4052 | |||||
| PE | GM-4053 | |||||
| Lactoferrin | Mouse | aC5 | IgG1 | Purifed | GM-4111 |
Intracellular expression in specific granules. Identifies leukocytes with granulocytic differentiation, i.e. neutrophils. |
| PE | GM-4113 | |||||
| Myeloperoxidase | Mouse | 8E6 | IgG1 | Purified | GM-4191 |
Identifies cells with myeloid differentiation, with low expression levels in monocytes. Represents the most important myeloid lineage marker. |
| (MPO-C2) | FITC | GM-4192 | ||||
| PE | GM-4193 | |||||
| Lysozyme | Mouse | LZ-2 | IgG1 | Purified | GM-4131 |
Identifies leukocytes with myeloid (monocytic) differentiation (monocyte lineage marker). Strongly positive in monocytes. |
| FITC | GM-4132 | |||||
| PE | GM-4133 | |||||
| CD68 | Mouse | Ki-M7 | IgG1 | FITC | GM-4152 |
Identifies monocytes (strong cytoplasmic) and macrophages, also expressed in subsets of B cells. |
| IL-1ß | Mouse | FIB3 | IgG1 | FITC | GM-4162 |
Major effector cytokine of monocytes/macrophages, induced by e.g. bacterial products (LPS), for intracellular detection of IL-1β. |
| IFN-γ | Mouse | GZ4 | IgG1 | FITC | GM-4172 |
Major effector cytokine of T helper 1 CD4+ T cells, for intracellular detection of IFN-γ. |
| COMBI-IC Antibodies/Conjugates | ||||||
| Specificity | Host | Clone | Isotype | Format | Product Code. | Application |
| Negative Control | Mouse | VI-AP | IgG1 | FITC | GIC-201 |
Non-binding isotype control antibody, for determination of non-specific binding of Ab via e.g. Fc-receptors to cell population of interest |
| Mouse | IgG1 | PE | ||||
| Lysozyme | Mouse | LZ-2 | IgG1 | FITC | GIC-206 |
Combi-reagent differentiates between granulocytic (LZ+/LF+) and monocytic (LZ+/LF-) cell populations in e.g. bone marrow aspirates, peripheral blood. |
| Lactoferrin | Mouse | 4C5 | IgG1 | PE | ||
| Myeloperoxidase (MPO-C2) | Mouse | 8E6 | IgG1 | FITC | GIC-212 |
Combi-reagent differentiates between granulocytic (MPO+/LF+) and monocytic (MPO+/LF-) cell populations in e.g. bone marrow aspirates, peripheral blood. |
| Lactoferrin | Mouse | 4C5 | IgG1 | PE | ||
| Myeloperoxidase (MPO-C2) | Mouse | 8E6 | IgG1 | FITC | GIC-213 |
Combi-reagent differentiats between myeloid lineage (MPO+/CD3-) and T cell committed (MPO- /CD3+) cell populations in e.g. leukemia screening/typing of bone marrow aspirates, peripheral blood. |
| CD3 | Mouse | UCHT1 | IgG1 | PE | ||
| Myeloperoxidase (MPO-C2) | Mouse | 8E6 | IgG1 | FITC | GIC-214 |
Combi-reagent differentiates between myeloid lineage (MPO+/CD22-) and B cell committed (MPO- /CD22+) cell populations, e.g. in leukemia typing of bone marrow aspirates, peripheral blood |
| CD22 | Mouse | RFB4 | IgG1 | PE | ||
| Single Surface Antibodies/Conjugates | ||||||
| Specificity | Host | Clone | Isotype | Format | Product Code. | Application |
| CD3 | Mouse | UCHT1 | IgG1 | Purifed | GM-4011 |
Multigene surface receptor structure representing the signaling-sheath of the T cell antigen receptor (TCR). Identifies and enumerates mature human T lymphocytes (mature T lineage marker) and a subset of thymocytes |
| FITC | GM-4012 | |||||
| PE | GM-4013 | |||||
| CD22 | Mouse | RFB4 | IgG1 | Purifed | GM-4051 |
Identifies and enumerates mature B lymphocytes. Surface-expressed on mature B-cells (B lineage marker) |
| FITC | GM-4052 | |||||
| PE | GM-4053 | |||||
| CD45 | Mouse | VIT200 | IgG1 | Purifed | GM-4061 |
Pan-leukocyte marker molecule. Identifies hematopoietic cells, is down-regulated on distinct leukemia blasts, is therefore used as gating-marker for blasts in leukemia screening/typing. |
| FITC | GM-4062 | |||||
| CD14 | Mouse | MEM18 | IgG1 | Purifed | GM-4091 |
Monocyte differentiation antigen, highly expressed on mature monocytes (with low expression levels also on neutrophils) (monocyte lineage marker) |
| FITC | GM-4092 | |||||
| PE | GM-4093 | |||||
| CD65s | Mouse | VIM2 | IgG1 | Purifed | GM-4101 |
Fucogangliosid, identifies acute myeloid leukemia cells and distinct pre-B ALL cells. Also expressed on normal granulocytes and monocytes. |
| FITC | GM-4102 | |||||
| CD117 (c-kit) | Mouse | 95C3 | IgG1 | Purifed | GM-4141 |
Stem cell factor receptor, used to detect hematopoietic progenitor cells (most lineages) and mast cells. |
| PE | GM-4143 | |||||
| TCR α/β | Mouse | BMA031 | IgG1 | PE | GM-4183 |
Pan T cell antigen receptor antibody, identifies mature T cells expressing the alpha/beta TCR (>90% of peripheral blood T cells). |
| Combi Surface Antibodies/Conjugates | ||||||
| Specificity | Host | Clone | Isotype | Format | Product Code. | Application |
| COMBI Gate Control | GCT-201 |
Combi-reagent identifies leukocytes (CD45+) and differentiates them from monocytes (CD14+) used as gating control to unequivocally identify major cellular populations (lymphocytes, monocytes, granulocytes) in whole blood, bone marrow etc. |
||||
| CD45 | Mouse | VIT200 | IgG2a | FITC | ||
| CD14 | Mouse | MEM18 | IgG1 | PE | ||
| COMBI Negative | GCT-202 |
Combi-reagent used as negative control to identify non-specific binding of antibodies to cell types of interest (e.g. via Fc-receptors). |
||||
| Control | Mouse | IgG2a | FITC | |||
| Mouse | IgG1 | PE | ||||
| Isotype Controls | ||||||
| Specificity | Host | Clone | Isotype | Format | Product Code. | Application |
| Negative Control | Mouse | IgG2a | FITC | GM-4982 |
Negative control to identify non-specific binding of respective antibody subclass to cell types of interest (e.g. via Fc-receptors). |
|
| Negative Control | Mouse | IgG2a | PE | GM-4983 | ||
| Negative Control | Mouse | IgG1 | Purified | GM-4991 | ||
| Negative Control | Mouse | IgG2 | FITC | GM-4992 | ||
| Negative Control | Mouse | IgG3 | PE | GM-4993 | ||
And remember, if you can't find what you're looking for - give us a call and we'll do the hard work for you.
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